Table II.
Copper Effects on the Stability of PAI-1 in the Presence of the VN and SMB Domain
| Additive | Cofactor | klat (s−1) | t1/2 (h) | Fold stabilization |
|---|---|---|---|---|
| NaCl | None | 2.28 (± 0.08) × 10−4 | 0.85 (± 0.03) | NA |
| VN | 1.33 (± 0.04) × 10−4 | 1.45 (± 0.04) | 1.71 (± 0.08) | |
| SMB | 1.18 (± 0.03) × 10−4 | 1.64 (± 0.04) | 1.94 (± 0.08) | |
| CuCl2 | None | 3.77 (± 0.14) × 10−4 | 0.51 (± 0.02)a | NA |
| VN | 0.63 (± 0.06) × 10−4 | 3.08 (± 0.27)b | 6.03 (± 0.58) | |
| SMB | 0.74 (± 0.05) × 10−4 | 2.62 (± 0.17)c | 5.13 (± 0.39) |
a
Significantly different from PAI-1 chloride control (P < 0.05).
b
Significantly different from PAI-1 + VN chloride control (P < 0.05).
c
Significantly different from PAI-1 + SMB chloride control (P < 0.05).