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Calculated Dissociation Constants for Metal/Buffer Binding Interactions
| Metal | Buffer | β1a | Ka (M−1) | Kd (μM) |
|---|---|---|---|---|
| Magnesium | MOPS | 3.51 | 3.24 × 103 | 309 |
| Manganese | MOPS | 3.54 | 3.47 × 103 | 288 |
| Cobalt | MOPS | 3.41 | 2.57 × 103 | 389 |
| Nickel | MOPS | 3.45 | 2.82 × 103 | 355 |
| Nickel | Tris | 2.74 | 5.50 × 102 | 1820 |
| Copper | Tris | 4.05 | 1.12 × 104 | 89.1 |
