Table III.
Comparison of kobs,final from Approach-to-Equilibrium Changes in PAI-1 Intrinsic Protein Fluorescence with klat Measured from Kinetics Measuring the Half-Life of PAI-1 Using Similar Conditions for Buffer, pH, and Temperature
| Metal | Buffer | Total metal | Free metal | kobs final (s−1) | klat (s−1) |
|---|---|---|---|---|---|
| None | MOPS | NA | NA | NA | 8.42 (±0.20) × 10−5 |
| Cobalt | MOPS | 20 mM | 250 μM | 3.09 (±0.02) × 10−4 | 3.42 (±0.07) × 10−4 |
| Nickel | MOPS | 20 mM | 230 μM | 2.17 (±0.04) × 10−3 | 1.89 (±0.13) × 10−3 |
The values for kobs final come directly from the multiple exponential fitting, whereas that for klat is the mean and standard error for three replicate experiments.