Skip to main content
. 2011 Feb 10;10:6. doi: 10.1186/1475-2859-10-6

Table 1.

Bacterial strains, plasmids and oligonucleodide primers used in this study.

Description Contents Reference/source
Strains
E. coli TG1 supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK- mK-) [F' traD36 proAB lacIqZΔM15 Stratagene
B. subtilis 1012 leuA8, metB5, hsrM1, nonA CatchMaps BV, MoBiTec
Plasmids
pET28a/sbpA/bet v1 Cloning of rsbpA/bet v1 [15]
pHT01 Ampr, Cmr, Pgrac promoter (consisting of groEpromotor, the lacO operator and the gsiBSD sequence, ColE1 ori,, lacI repressor, E. coli/B. subtilis shuttle vector MoBiTec
pHT43 see pHT01 + amyQ signal sequence MoBiTec
pHT01/sbpA/bet v1 Expression of rSbpA/Bet v1 in cytoplasm of B. subtilis 1012 This study
pHT43/sbpA/bet v1 Expression of rSbpA/Bet v1 in cytoplasm of B. subtilis 1012 followed by subsequent secretion into the culture medium This study
Primers*
SbpA/AatII/forward 5'-cgcgacgtcgcgcaagtaaacgactataacaaatc-3' This study
Bet v1/SmaI/reverse 5'-tcccccgggttagttgtaggcatcggagtgtg-3' This study

*Bold nucleotide sequences indicate restriction endonuclease sites.