Table 1.
Oligonucleotides used in this study
| Aim of primers | Name | Sequence (5'→3') |
|---|---|---|
| For csnN106 coding region cloning* | FwcsnN106 | CCGGAGACCCGCATGCCCCGGAC |
| RvcsnN106 | CGGTGCGCCAAGCTTGCGTTCGG | |
| For Pr-WT cloning* | FwPr-WT | GTCTGCGCGGATCCTGACGGCCC |
| RvPr-WT | GTCCGGGGCATGCGGGTCTCCGG | |
| PCR-directed mutagenesis for Pr-Pa cloning** | SEQ.1 | ACAACTTCGTCGCGCACATCCA |
| Rw1Pr-Pa | ATGAGGAGAGTTCGGACAGTTTC | |
| Fw2Pr-Pa | GAAACTGTCCGAACTCTCCTCAT | |
| RvcsnN106 | TGAGGTCGAAGTTCTTGGCGTT | |
| Verification of pHM8a derivatives integration into hosts | Fwgenom | CCTGAGAGGCCGGTGAGGAG |
| RvcsnN106 | TGAGGTCGAAGTTCTTGGCGTT | |
| Presence verification of pFDES derivatives into hosts | SEQ.1 | ACAACTTCGTCGCGCACATCCA |
| T7 promoter | TTAATACGACTCACTATAGGG | |
| For Primer extension | PE-csnN106 | TGGGGTGCTTGAGACGCAT |
*Bold nucleotides correspond to restriction site
**Bold nucleotide correspond to mutated nucleotide