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Immunoblot of hmunc13 and the C1-less mutant. hmunc13-HA (Hmunc13), C1-less mutant (C1 less), or empty plasmid pCMV·SPORT (PCMV) were transiently transfected into OK cells. Whole-cell lysates were prepared and subjected to 6% SDS-PAGE. The blot was detected by anti-HA. Note the slightly decreased molecular weight of the C1-less mutant.
