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Immunoblots of whole-cell lysates (A) and Golgi membrane preparations (B) from hmunc13-transfected OK cells with (+) or without (−) PDBu treatment for 3 h. The whole-cell lysates represent small aliquots of cells for Golgi membrane preparations. Equal amounts of protein were loaded onto each lane of A or B. The blots were then detected by anti-HA antibody.
