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. 1999 May;10(5):1609–1619. doi: 10.1091/mbc.10.5.1609

Figure 6.

Figure 6

Double labeling of apoptotic cells and expression of hmunc13 or C1-less mutant. hmunc13 (A–C and E–G) and C1-less mutant (D and H) transiently transfected cells were subjected to TUNEL labeled with fluorescein (E–H) and then subjected to anti-HA and anti-mouse IgG–rhodamine labeling for expression of hmunc13 and C1-less mutant (A–D). Cells were treated with vehicle (A and E) or 0.1 μM PDBu for 8 h (B, D, F and H) or 16 h (C and G). C1-less mutant-transfected cells treated with vehicle exhibit an image similar to D and H (our unpublished results). Negative controls of TUNEL by incubating cells with labeling mix and no TdT yielded no staining of fluorescein (our unpublished results). Arrowheads indicate representative cells costained with anti-HA (A–D) and TUNEL (E–H) from identical fields.