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. 2011 Feb 22;9:6. doi: 10.1186/1477-3155-9-6

Figure 4.

Figure 4

Activation of the apoptosis signal pathway. (A) Western blotting in vitro. Cell lysates were prepared from the cells treated with 50 μM ruthenium (II)-arene complex, 100 μM ruthenium (II)-arene complex. HCC827 cells without treatment were used as a control. The following antibodies were used: anti-cleaved Caspase-8, anti-cleaved Caspase-9, anti-cleaved Caspase-3, and anti-PARP antibody. GAPDH was served as a loading control. (B) Western blotting in vivo. Tumor lysates were prepared from the cells treated with 50 μmol/kg ruthenium (II)-arene complex, 100 μmol/kg ruthenium (II)-arene complex. HCC827 cells without treatment were used as a control. The following antibodies were used: anti-cleaved Caspase-8, anti-cleaved Caspase-9, anti-cleaved Caspase-3, and anti-PARP antibody. GAPDH was served as a loading control.