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. 2011 Feb 11;11:43. doi: 10.1186/1471-2148-11-43

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Copyright ©2011 Haiko et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of expression of Pla-Epo hybrid proteins in E. coli. Expression of the Pla-Epo-hybrid proteins was analyzed by Western blotting of whole cells with a mixture of anti-Pla, anti-Epo, and anti-Pla-loop antisera. The constructs are indicated with their plasmid names (see Table 1). Migration distance of the Pla isoforms are indicated; pre-Pla is the immature form of Pla, α-Pla and γ-Pla are mature Pla proteins with differing conformations, and β-Pla is the mature autocleaved form of Pla.