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Degradation of serpins by E. coli expressing the Pla-Epo hybrid proteins. Degradation of PAI-1 (A) and α2AP (B) in a 2-h incubation was analyzed by Western blotting. The black arrowhead indicates uncleaved serpin and the white arrowhead indicates the degradation product. The strains are indicated with their plasmid names (see Table 1).
