Table 1.
Overview concerning anamnestic data of the cows and immunohistochemical results of all samples examined
| Months p.i. | Cow | Status | PrPSc in the Obex (IHC) | Ileum | Ileocaec.-junct. | Jejunum | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| No. pos. Foll./Total No. Foll. | No. pos. Foll. in % | TBM/Intracellular Reaction Pattern | FDC | ENS | No. pos. Foll./Total No. Foll. | No. pos. Foll./Total No. Foll. | ||||
| 1 (n = 3) | IT 04 | preclinical | negative | 0/9189 | 0 | -- | -- | -- | 0/3289 | 0/1337 |
| IT 44 | preclinical | negative | 0/6272 | 0 | -- | -- | -- | 0/912 | 0/761 | |
| IT 62 | preclinical | negative | 0/4634 | 0 | -- | -- | -- | 0/721 | 0/9479 | |
| 4 (n = 2) | IT 19 | preclinical | negative | 1/4076 | 0.02 | punctate | -- | -- | 0/102 | 0/848 |
| IT 45 | preclinical | negative | 4/4399 | 0.09 | punctate | -- | -- | 0/162 | 0/1453 | |
| IT 14 | preclinical | negative | 0/726 | 0 | -- | -- | -- | 0/394 | 0/1256 | |
| 8 (n = 4) | IT 20 | preclinical | negative | 15/2554 | 0.6 | punctate/fine granular | -- | -- | 0/1037 | 0/2636 |
| IT 39 | preclinical | negative | 1/1309 | 0.07 | punctate | -- | -- | 0/601 | 0/457 | |
| IT 55 | preclinical | negative | 1/2285 | 0.04 | punctate | -- | -- | 0/279 | 0/475 | |
| 12 (n = 4) | IT 01 | preclinical | negative | 89/461 | 19.3 | punctate/fine granular | (+)* | -- | 0/352 | 0/437 |
| IT 16 | preclinical | negative | 3/1711 | 0.2 | punctate | -- | -- | 0/1252 | 0/1135 | |
| IT 06 | preclinical | negative | 56/957 | 5.8 | fine granular/globular | (+)* | --. | 3/418 | 0/201 | |
| IT 57 | preclinical | negative | 65/1384 | 4.7 | fine granular/globular | (+)* | -- | 0/1251 | 0/495 | |
| 16 (n = 4) | IT 07 | preclinical | negative | 0/135 | 0 | -- | -- | -- | 0/909 | 0/325 |
| IT 28 | preclinical | negative | 4/1514 | 0.3 | fine granular/globular | + | + | 6/1149 | 0/550 | |
| IT 46 | preclinical | negative | 0/708 | 0 | -- | -- | -- | 0/510 | 0/970 | |
| IT 65 | preclinical | negative | 8/2483 | 0.3 | fine granular | (+) | -- | 0/975 | 0/1112 | |
| 20 (n = 4) | IT 10 | preclinical | negative | 0/774 | 0 | -- | -- | -- | 0/580 | 0/1187 |
| IT 17 | preclinical | negative | 1/1369 | 0.07 | fine granular/globular | -- | -- | 26/1456 | 0/1004 | |
| IT 50 | preclinical | negative | 1/611 | 0.2 | globular | -- | -- | 0/449 | 0/712 | |
| IT 60 | preclinical | negative | 2/902 | 0.2 | fine granular/globular | + | -- | 0/699 | 0/265 | |
| 24 (n = 4) | IT 26 | preclinical | positive | 35/389 | 9.0 | globular | + | +++ | 0/819 | 0/996 |
| IT 24 | preclinical | negative | 29/438 | 6.6 | globular | + | + | 0/1037 | 0/1999 | |
| IT 47 | preclinical | negative | 89/343 | 25.9 | fine granular | (+) | + | 0/511 | 0/1114 | |
| IT 58 | preclinical | negative | 0/86 | 0 | -- | -- | -- | 4/1027 | 0/1473 | |
| 28 (n = 4) | IT 08 | preclinical | positive | 6/508 | 1.2 | fine granular | (+) | + | 0/182 | n.d. |
| IT 21 | preclinical | positive | 4/489 | 0.8 | fine granular | -- | -- | n.d. | n.d. | |
| IT 51 | preclinical | negative | 1/854 | 0.1 | fine granular | -- | -- | 0/669 | n.d. | |
| IT 52 | preclinical | negative | 2/1574 | 0.1 | punctate | -- | -- | n.d. | n.d. | |
| 32 (n = 4) | IT 05 | preclinical | positive | 0/114 | 0 | -- | -- | + | 0/1278 | n.d. |
| IT 09 | clinical | positive | 10/54 | 18.5 | fine granular/globular | + | +++ | n.d. | n.d. | |
| IT 43 | clinical | positive | 11/694 | 1.6 | fine granular/globular | (+) | -- | 0/569 | n.d. | |
| IT 61 | clinical | positive | 7/291 | 2.4 | fine granular/globular | (+) | -- | n.d. | n.d. | |
| 36 (n = 4) | IT 49 | clinical | positive | 12/314 | 3.8 | fine granular | (+) | + | n.d. | n.d. |
| IT 48 | preclinical | negative | 2/473 | 0.4 | globular | -- | -- | 0/1306 | n.d. | |
| IT 11 | clinical | positive | 0/349 | 0 | -- | -- | ++ | n.d. | n.d. | |
| IT 23 | clinical | positive | 14/177 | 7.9 | globular | + | +++ | 0/602 | n.d. | |
| 40 (n = 4) | IT 13 | clinical | positive | 15/385 | 3.9 | fine granular/globular | (+)* | ++ | 0/750 | n.d. |
| IT 25 | clinical | positive | 0/45 | 0 | -- | -- | ++ | n.d. | n.d. | |
| IT 56 | preclinical | positive | 1/661 | 0.2 | fine granular | -- | + | n.d | n.d. | |
| IT 41 | preclinical | positive | 0/112 | 0 | -- | -- | +++ | 0/564 | n.d. | |
| 44 (n = 4) | IT 15 | clinical | positive | 1/121 | 0.8 | fine granular | -- | + | 0/546 | 453 |
| IT 22 | clinical | positive | 0/215 | 0 | -- | -- | +++ | n.d. | n.d. | |
| IT 02 | clinical | positive | 0/131 | 0 | -- | -- | ++ | 1*/724 | 859 | |
| IT 53 | clinical | positive | 3/480 | 0.6 | fine granular/globular | -- | + | 0/690 | 1390 | |
| IT 38 | clinical | positive | 0/155 | 0 | -- | -- | -- | n.d. | n.d. | |
TBM: Tingible Body Macrophages, n.d.: not done, * only seen with mab 6C2, pos: positive, Foll: Follicle
ENS: total number of positive labelled foci in the ENS (submucosal and myenteric plexuses), +:0-4 positive foci in the ENS, ++: 5-10 positive foci in the ENS, +++: > 11 positive foci in the ENS
FDC: (+): weak dendritic network, +: clear dendritic network