Table 3.
Bioassay of different localizations of bovine small intestine in bovine transgenic mice (Tgbov XV): results (revealed by PTA-Immunoblot), transmission rates and incubation periods
| Months p.i. | Cow | Localization | |||
|---|---|---|---|---|---|
| Jejunum A | Jejunum B | Ileum | Ileocaec. | ||
| IT 14 | 0/10 -- |
0/14 -- |
9/13 280 ± 41 |
0/15 -- |
|
| 8 | IT 20 | 0/14* -- |
0/13 -- |
8/11 334 ± 61 |
0/15* -- |
| IT 39 | 1/14* 502 |
0/12* -- |
7/11* 310 ± 49 |
0/12 -- |
|
| IT 55 | 2/10* 379, 522 |
0/12 -- |
7/9 332 ± 47 |
3/13 442, 517, 582 |
|
| 12 | IT 01 | 0/14* -- |
3/10 407, 432, 600 |
12/13 305 ± 45 |
14/15 315 ± 40 |
| IT 16 | 0/13 -- |
0/12 -- |
6/11 412 ± 31 |
1/10 395 |
|
| IT 06 | 0/12 -- |
1/13 375 |
10/10 279 ± 47 |
12/12 277 ± 39 |
|
| IT 57 | 0/15 -- |
2/10 393, 428 |
11/11 260 ± 55 |
9/11 297 ± 64 |
|
| IT 07 | 0/14* -- |
0/15 -- |
0/12* -- |
0/9 -- |
|
| 16 | IT 28 | 0/11 -- |
0/11 -- |
0/11 -- |
0/12 -- |
| IT 46 | 0/11 -- |
0/12 -- |
0/10 -- |
0/12 -- |
|
| IT 65 | 0/12 -- |
0/11 -- |
11/14* 319 ± 41 |
13/13* 349 ± 53 |
|
| 20 | IT 10 | 0/13 -- |
2/15 363, 408 |
0/11* -- |
0/9 -- |
| IT 17 | 0/12* -- |
0/10 -- |
0/15* -- |
12/12 311 ± 33 |
|
| IT 50 | 0/12* -- |
0/14* -- |
11/13 353 ± 57 |
0/12* -- |
|
| IT 60 | 1/12 391 |
0/9 -- |
11/12 345 ± 37 |
0/12 -- |
|
The first line indicates the number of positive tested mice/total number of mice, the second line indicates the mean incubation period in days post infection
* augmented groups - additional inoculations after heating the inoculum to 70°C for 10 min