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Characterization of recombinant DICER protein. Purified rDICER (3 μg) was loaded onto a NuPAGE 4-12% Bis-Tris gel. Proteins were analyzed by Coomassie Brilliant Blue (CBB) staining (A) and Western blotting with anti-DICER antibody (B). (C) Western blotting with anti-DICER, anti-FLAG, anti-AGO2 and anti-TRBP antibodies. 30 μg of 293T cell lysate (lane 1) and 3 μg of rDICER (lane 2) were loaded.
