Table 1.
Primer sequences, amplicon lengths and reaction efficiencies in RT-qPCR study.
| Symbol | Primer sequence (5'-3') |
size (bp) |
Tm (°C) |
Primer |
E (error) |
SD(E) |
|---|---|---|---|---|---|---|
| 18SrRNA | F: CTGAGAAACGGCTACCACATC R: ACCAGACTTGCCCTCCAAT |
171 | 84.9 | 0.25 | 1.91 (0.02) |
0.02 |
| Arm | F: ACTTCTTATGAGAGCATTCCAGGAT R: CCTTCAACAATTTCTTCCATGC |
114 | 83.2 | 0.3 | 1.81 (0.03) |
0.01 |
| EF1a | F: AGCCCAGGAGATGGGTAAAG R: CTCTGTGGCCTGGAGCATC |
155 | 81.4 | 0.3 | 1.99 (0.08) |
0.04 |
| RpL32 | F: ACTGGAAGTCTTGATGATGCAG R: CTGAGCCCGTTCTACAATAGC |
97 | 78.6 | 0.25 | 1.97 (0.05) |
0.04 |
| GAPDH | F: AATTGCCTGGCACCATTG R: CGCCACAACTTTCCAGATG |
128 | 80.7 | 0.3 | 1.95 (0.06) |
0.00 |
| Actin | F: TTGTGTTGGATTCTGGTGATG R: GAAGCTGTAGCCCCTCTCAG |
149 | 83.5 | 0.5 | 1.66 (0.01) |
0.02 |
| SDHa | F: CCACTGAAACTGATCCAAGAGAG R: TCCTGCTCCATTAACTAAGCAAC |
98 | 76.2 | 0.3 | 1.90 (0.05) |
0.10 |
| AnnIX | F: GGAACTGATGAGGAAGCCATT R: TGGCCTGAAGTGTCTCCTTT |
134 | 77.2 | 0.5 | 1.91 (0.04) |
0.05 |
size: size of the cDNA amplicon; Tm: melt temperature of the PCR amplicon averaged over the 15 biological samples and 3 technical replicates; Primer: primer concentration in μM; E: in-run PCR reaction efficiency (fold increase per cycle) computed from the standard curve of a serial dilution included in the same plate as for samples S1 to S15 and using the formulae 10-(1/-slope of the standard curve); error: the mean square error of the standard curve; SD(E): standard deviation of the PCR efficiencies estimated from duplicated standard curves (different RT-qPCR runs and cDNA templates).