Table 1.

Primers used for quantitative polymerase chain reaction

Gene Name	Oligo Name	Sequence	Tm (°C)	Length (nt)
GAPDH	mrGapdh-top	TTGTCAGCAATGCATCCTGCACCACC	61	119
	mrGapdh-rev2bot	CTGAGTGGCAGTGATGGCATGGAC	61
3'-terminal exons
Kalirin7-specific	KalEx33-for	GATACCATATCCATTGCCTCCAGGACC	61	127
	Kal7unique-rev	CCAGGCTGCGCGCTAAACGTAAG	62
Kalirin8-specific	KalEx38-for	CTGACGTGCCTACTGCTGCAGAC	61	130
	Kal8unique-rev	CTGGTTGAGGTCCTGGGAGCCAC	62
Kalirin9-specific	KalEx51-for	GCCCCTCGCCAAAGCCACAGC	62	125
	Kal9unique-rev	CCAGTGAGTCCCGTGGTGGGC	62
Kalirin12-specific	KalEx62-for	CAGCAGCCACGTGCCTGCAGC	62	140
	KalEx62-rev	TCTTGACATTGGGAATGGGCCGCAC	61
Promoter-specific
Kalirin A promoter	KalPromA-for	CAGGGCAGCCATAAATGGTTTTATCTG	58	121
	KalEx3-rev	CCTCGCTTGTCACGCCCCC	60
Kalirin B promoter	KalPromB-for	GTGGACGCCTTTTTCCGGACAG	59	116
	KalEx3-rev	CCTCGCTTGTCACGCCCCC	60
Kalirin C promoter	KalPromC-for	CTTGCTTCGGCTTCTGGATCGAG	59	117
	KalEx3-rev	CCTCGCTTGTCACGCCCCC	60
Kalirin D promoter	KalPromD-for	CTTTGTTTCTTCCTCACTGCAGCGG	59	120
	KalEx3-rev	CCTCGCTTGTCACGCCCCC	60
ΔKalirin promoter	ΔKalProm-for	ATCCCAGGGTGAGCAGTGAGAAGG	61	144
	mKal-Ex11-rev	ATTCCCCAGTCTGAGCCAGCTGC	61
Full-length Kalirins	mKal-Ex10-for	GCCTTTCTCAGCAAACACACTGGGG	61	157
	mKal-Ex11-rev	ATTCCCCAGTCTGAGCCAGCTGC	61