Figure 2.

Blocking protein synthesis diminishes substantial gene inductions by in-polyIC stimulation. HT1080 cell line was treated with 40 μg/ml ex-polyIC and 0.4 μg/ml in-polyIC for 8 hours in the presence/absence of 10 μg/ml cycloheximide. Gene expression was measured using qPCR and the mean from triplicate experiments was calculated. Error bars represent standard error. Gene inductions in response to ex-polyIC are not significantly affected by cycloheximide treatment whereas gene inductions in response to in-polyIC are diminished by cycloheximide treatment.