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. 2011 Feb 17;12:16. doi: 10.1186/1471-2172-12-16

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Copyright ©2011 Liao et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Construction of pEGFP-3CL^proand pEGFP-C145A expression vectors. (A) Scheme of pEGFP-3CL^proand pEGFP-C145A construction. PCMV, human cytomegalovirus (CMV) immediate early promoter; EGFP, enhanced green fluorescent protein gene; MCS, multiple cloning sites; 3CL^pro, SARS-CoV 3CL^progene; C145A, 3CL^proC145A mutant gene; myc, myc-tag sequence; pA, SV40 early mRNA polyadenylation signal sequence. (B)The constructs were digested with restriction enzyme and separated on 1% agarose gel. M, DNA markers.