Table 3.

Amino acid changes during consecutive passages of rgVac1 mutants

Passage number	rgVac1sub (H5N1)									rgVac1ins (H5N1)
	PA		HA		NP		M1		M2		HA		NA	M1
	65a	672	308	338	213	374	89	101	45	157	298	465	171	130
P0	S	L	H	-	R	M	D	R	R	S	M	D	N	L
P1	.b	.	Q	Rc	.	.	.	.	H	P	.	E	.	.
P2	T	.	Q	R	.	.	.	K	H	P	.	.	.	.
P3	.	F	Q	R	Q	V	N	K	H	P	I	.	N/Hd	I

^a Methionine encoded by the AUG start codon is defined as position 1.

^b Periods indicate same amino acids as the parental virus.

^c Arginine was inserted at the HA cleavage site.

^d Amino acid quasispecies were observed.