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. 2011 Feb 24;434(Pt 3):427–434. doi: 10.1042/BJ20101813

Figure 3. Binding of TRAP to RNA at different temperatures.

Figure 3

The data from EMSA (electrophoretic mobility-shift assays) experiments were fitted to a 1:1 binding equation. The x-axis represents the concentration of the 11-mer TRAP ring, whereas the y-axis denotes the percentage of the fluorescent signal that is shifted. Black, 10 °C; grey, 20 °C; blue, 30 °C; green, 40 °C; orange, 50 °C; and red, 60 °C. (A) TRAPsub without L-Trp; (B) TRAPsub with L-Trp; (C) TRAPste without L-Trp; and (D) TRAPste with L-Trp.