Fig. 2.

Changes in cytosine–guanine dinucleotide (CpG)-induced immunoglobulin production in patients with Kawasaki disease (KD) in acute phase versus healthy controls. (a) Peripheral blood mononuclear cells (PBMC) from 10 acute-phase KD patients and 10 age-matched controls were stimulated with CpG oligodeoxynucleotide (ODN) and after 7 days immunoglobulin (Ig)M-, IgG- and IgA-secreting cells (SC) were enumerated by enzyme-linked immunospot (ELISPOT) assay. Comparisons by unpaired t-test revealed significant differences in the number of IgA-SC between the control (CTR) and KD groups. P-values <0·05 were considered significant. (b) Number of IgM-, IgG- and IgA-SC in two KD patients before (pre-IVIG) and after (post-IVIG) therapy. Mean ± standard error of the mean from 10 age-matched CTR subjects are reported.