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RT-PCR analysis of the DVU_0377 (A), DVU_1838 (B), and DVU_1457 (C) gene clusters. RNAs were extracted, and after cDNA synthesis with reverse transcriptase, cDNAs were further amplified in PCR by addition of primer pairs (supplemental Table S1) to reveal transcriptional links between successive genes. The PCR products were analyzed by electrophoresis on agarose gel. −, PCRs performed on RNAs; +, PCRs performed on genomic DNA; c, PCRs performed on cDNAs. M, DNA size marker.
