FIGURE 1.

Induction of TAp73 expression by NO in K-562 cells. A, cells were treated for 6–48 h with 0.5 mm DETA-NO, and TAp73α protein levels were analyzed by immunoblotting using β-actin as a loading control. B, dose-dependent induction of TAp73α protein expression in K-562 cells stimulated for 24 h with the indicated concentrations of DETA-NO and SNAP are shown. C, cells were treated as stated in B, and TAp73 mRNA expression levels were determined by real-time PCR relatively to untreated controls. Significant differences of p < 0.05 (*) or 0.01 (**) versus control cells were calculated by using Student's t test.