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Identification of the LipL product. HPLC analysis of the LipL reaction using authentic UMP without α-KG (I), a 15-min reaction containing all of the necessary components (II), the 15-min reaction mixture spiked with synthetic uridine-5′-aldehyde (III), and synthetic uridine-5′-aldehdye (IV) are shown. A260, absorbance at 260 nm.
