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Replacing C20 hydrogen atoms of all-trans-retinaldehyde with deuteriums slows the formation of A2E. A, HPLC profiles of reaction mixtures containing C20-D3-retinaldehyde or retinaldehyde, plus ethanolamine and acetic acid after 30 h. B, expanded section of panel A. Gray line: retinaldehyde reaction; Black line: C20-D3-retinaldehyde reaction. C, plots of A2E formation over time for the reactions in panel A (kH/kD = 7 ± 1; from an average of three runs).
