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Administration of C20-D3-vitamin A to wild-type mice slows A2E synthesis in vivo. A, representative HPLC trace of extracted retinoids from eyecups of mice administered C20-D3-vitamin A. B and C, UV-Vis absorbance spectra of the peaks at B ∼10.8, representative of A2E and C, ∼11.8 min, representative of iso-A2E. D, average A2E levels in animals given either vitamin A at natural abundance or C20-D3-vitamin A. Five animals per group were used. p: p value.
