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. 2010 Dec 30;286(10):7990–7999. doi: 10.1074/jbc.M110.163006

FIGURE 3.

FIGURE 3.

CaMKIIδ deletion affects the expression of cell cycle regulators after carotid artery ligation. A, ligated and contralateral unligated carotid arteries were explanted 14 days after ligation. 10 μg of lysate was resolved by SDS-PAGE and blotted with antibodies as indicated. (n = 6–8 mice per group). B, quantitation of three independent experiments by densitometric analysis (*, p < 0.05). C, cdk2, cyclinD1, and E, p21 immunofluorescence (green) in C57Bl/6 littermate control and CaMKIIδ−/− mouse carotid artery 14 days after ligation (smooth muscle-actin-red, DAPI-blue, scale bar 25 μm). Inset: detail of the neointima and media of a ligated control and of the media of a ligated CaMKIIδ−/− carotid artery. D, densitometric quantitation of cdk2, cyclinD1, and E, p21 in the neointima and media of the ligated control and in the media of the ligated CaMKIIδ−/− carotid artery, adjusted for area and background. Because no measurable neointima developed after ligation in CaMKIIδ−/− carotid arteries, no densitometry of the CaMKIIδ−/− neointima is provided.