TABLE 3.

Comparison of the conductance between the WT-FhuA protein and its deletion mutants

The unitary conductance was obtained from single channel electrical recordings in 1 m KCl, 10 mm phosphate, pH 7.4. The applied transmembrane potential was +40 mV.

Protein	Conductance
	nS
WT-FhuA	0.3 ± 0.2 (n = 4)
FhuA Δ1–160	2.5 ± 0.6 (n = 4)
FhuA Δ335–355	3.1 ± 0.2 (n = 3)
FhuA Δ1–160/Δ335–355	3.0 ± 0.5 (n = 3)a
FhuA Δ1–160/Δ322–355	3.0 ± 1.5 (n = 4)b
mFhuA ΔC/Δ4L	4.8 ± 1.3 (n = 58)
rFhuA ΔC/Δ4L	4.9 ± 0.7 (n = 25)

^a The conductance measurements were derived from the peak that corresponds to the most probable conductance in all-points current amplitude histogram (Fig. 3A, right panel).

^b The conductance measurements were derived using the S₃ sub-state in the all-points current amplitude histogram (Fig. 3B, right panel).