FIGURE 4.

Independent binding of the human complement inhibitors C4BP and Factor H to C. albicans Pra1. Pra1 was immobilized, and a constant amount of Factor H (2.5 μg/ml) together with increasing concentrations of C4BP used at different mass ratios (1:0, 1:1, 1:2, 1:5, 1:10, and 1:20) were added. Bound Factor H (hatched bars) was detected with polyclonal goat Factor H antiserum, and bound C4BP (black bars) was detected with mouse C4BP antibody (mAb 67). Data shown represent the mean values ± S.D. (error bars) of three separate experiments.