TABLE 1.

Oligonucleotide primers used in RT-PCR assays

AFMID, arylformamidase; KYNU, kynureninase; KMO, kynurenine 3-hydroxylase; 3HAO, 3-hydroxyamino oxidase; KAT3, kynurenine aminotransferase.

Gene product	Primer sequences (5′ → 3′)	Product length	Tm
		bp	°C
IDO1-T1	GGGGGTCAGTGGAGTAGACA (forward)	173	60
	GCAGATTTCTAGCCACAAGGA (reverse)
IDO1-T2	TGCCTGGTTTTGAGGTTTTC (forward)	246	60
	GCAGATTTCTAGCCACAAGGA (reverse)
INDOL	CCTCATCCCTCCTTCCTTTC (forward)	218	57
	GGAGCAATTGCCTGGTATGT (reverse)
TDO	TGAATGCGCAAGAACTTCAG (forward)	244	58
	TTCCAGAACCGAGAACTGCT (reverse)
KYNU	CTTGGCCTTCAACCGAAA (forward)	200	57
	CAAAGCATTCATTAGAGCTATTTCTT (reverse)
KMO	TTCAATAAGCAGAGAAAACTTAAACAA (forward)	199	57
	TCACACCCTACAACAAGGTCA (reverse)
3HAO	CTAAGCCTGTTTGGGGACAG (forward)	197	60
	GGTTCCAGCAGGCACCAG (reverse)
AFMID	CCGCAGTGTCGTGTTTCTAC (forward)	200	60
	CTATGAGGGGCTCCAGGTC (reverse)
KAT3	GCTGCCAAGATGGTCTCTGT (forward)	200	57
	TTCCACTGGGAAAGGATTTTT (reverse)
GAPDH	GAAGACTGTGGATGGCCCCTC (forward)	358	58
	GTTGAGGGCAATGCCAGCCCC (reverse)