FIGURE 4.

Expression of different Gα_i/o class members but not Gα₁₂ rescues the impaired proliferation caused by Gα_i2/Gα_i3 knockdowns. Freshly isolated CGNPs were electroporated with mixtures of cDNA containing Gi2UTR2/Gi3UTR2 and constructs including wild-type (A) or active forms (B) of the different Gα_i/o class members. Cells were cultured in a saturating concentration of Shh (3 μg/ml) for 48 h, and proliferation was measured by BrdU incorporation. Selected images of transfected cells are shown in C. A more complete panel of images is presented in supplemental Fig. 3.