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. 2010 Dec 29;286(10):8106–8116. doi: 10.1074/jbc.M110.134692

FIGURE 1.

FIGURE 1.

Navβ2 is required for BACE1 activity-dependent regulation of Nav1.1 levels. Primary neuronal culture (14 days in vitro) from Navβ2-null mice (Navβ2 KO), wild-type control mice (WT), and wild-type rat were treated with control vehicle (DMSO) or BACE inhibitors (DR9 and/or inhibitor IV) for 48–72 h. Nav1.1 protein levels were analyzed by Western blot. A, upper panel, Nav1.1 Western blot analysis of rat primary neurons (14 days in vitro) treated with various concentrations of DR9 and control vehicle DMSO for 72 h; lower panel, quantitative analysis of Nav1.1 protein levels. B, representative Western blot showing changes in Nav1.1 levels upon BACE1 inhibitor treatment in cultured WT neurons. C, quantitative analysis of Nav1.1 protein levels upon BACE1 inhibitor treatment in cultured WT neurons. D, representative Western blot showing Nav1.1 levels of cultured Navβ2 KO neurons treated with DMSO, DR9, or inhibitor IV. E, quantitative analysis of Nav1.1 protein levels of cultured Navβ2 KO neurons treated with DMSO, DR9, or inhibitor IV. One-way ANOVA followed by a post hoc Tukey's test was used for statistical analysis (*, p < 0.05; n = 3 per each condition).