FIGURE 1.

MLV capsid positions CA82, CA109, CA110, and CA117 influence restriction mediated by TRIM5α variants that have distinct retroviral specificities. A, Western blot analysis of extracts from MDTF cells stably transduced with retroviral vectors expressing HA-tagged versions of the indicated TRIM5α variants using HA (top)- and actin (bottom)-specific antibodies is shown. All TRIM5α variants exhibited wild type levels of expression, except for huTRIM5α R332P, that was detected at slightly higher steady-state levels. B, MDTF cells expressing wild type (WT) or the indicated mutant forms of huTRIM5α or transduced with a control vector were challenged with serial 2-fold dilutions of MLV-based vectors harboring either a WT or the indicated mutated capsid. Infections were scored at 72 h post-infection by FACS. These results are representative of two independent experiments.