FIGURE 5.

HIV1 capsid positions CA83, CA89, CA120, and CA122 modulate TRIM5α restriction. A, MDTF cells expressing the indicated TRIM5α variant or transduced with an empty vector were challenged with three different doses (in duplicate) of HIV1 vectors harboring either a WT or the indicated mutant CA. Infections were scored 72 h post-infection by flow cytometry. Graphs shows results obtained with a single dose, but similar results were observed with the two other doses. These results are representative of at least three independent experiments. B, MDTF expressing wild type (left graph) or Y336K huTRIM5α (right graph) were challenged with serial 2-fold dilutions of HIV-1 GFP vectors in the presence (+CsA) or absence of the drug cyclosporine A (−CsA). Scoring of the infections and calculation of the -fold restriction were performed as described in Fig. 4.