Figure 2.
Expression of OGG1 mRNAs in various human tissues and cell lines. (A) Northern blot analysis. Total RNAs (16 μg each) extracted from various human tissues and Jurkat and HeLa S3 cells were electrophoresed, transferred onto nitrocellulose membrane, and probed with a 32P-labeled 1891-bp fragment of type 2a cDNA. (B) RT-PCR analysis. cDNAs were synthesized from total RNA prepared from cerebrum, kidney, fetal brain, and Jurkat cells, using the oligo(dT)18 primer. The cDNAs were amplified using a common 5′ primer (N1) and 3′ specific primer for each type of OGG1 mRNA (type 1: OG18; type 2: OG21). PCR products were analyzed by 1% agarose gel electrophoresis. The presence of the primers in the reaction mixture is shown by +. ▪, Type 1b; ♦, type 2b; ✖, type 2c mRNA. (C) RT-PCR analysis. The cDNAs were amplified using a common 5′ primer (OG7) and 3′ specific primer for each type of OGG1 mRNA (type 1: OG18; type 2: OG21). PCR products were analyzed by 2% agarose gel electrophoresis. ▪, Type 1b; ♦, type 2d; ✖, type 2e mRNA; ★, a 0.4-kbp band that may correspond to the PCR product form type 1c mRNA.