Table 2.
Relative specific content of marker proteins in subcellular fractions
| Marker | Whole | Nuclei | Mitochondria | Microsomes | Cytosol |
|---|---|---|---|---|---|
| Succinate–cytochrome c reductase | 5.6 | ND | 100a | 0.2 | ND |
| Lactate dehydrogenase | 25.3 | 0.3 | 2.3 | 5.1 | 100b |
| Anti-HA–reacting protein (p50) | 32.7 | 0.1 | 4.6 | 100c | 26.1 |
All the fractions are derived from MR-2a:HA cells. Each value is a mean of four independent experiments. ND, not detected.
a
172.8 μmol · min−1 · mg−1 of protein.
b
1166.0 μmol · min−1 · mg−1 of protein.
c
1.9 photo-stimulated luminescence (PSL)/μg of protein.