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. 2011 Jan 6;286(10):8141–8148. doi: 10.1074/jbc.M110.165233

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — PrP^res from soluble fractions passes through 300-kDa filters. A, analysis of PK-digested lysates before (total) or after ultrafiltration through 300-kDa or 100-kDa filters. Retentates (R; 1/10 and 9/10 were loaded) and the whole filtrates (F) were analyzed for PrP^res by immunoblotting. B, filtrate fractions obtained after ultrafiltration through 1,000-kDa and 300-kDa filters of PK-digested lysates. Fractions were centrifuged to separate the insoluble (pellet) and soluble (sup) materials. Insoluble (1/10) and the whole soluble fractions from the filtrates were analyzed for PrP^res. C, supernatants of centrifuged PK-digested lysates ultrafiltrated through the indicated filters. PrP^res in the retentates (R) and the filtrates (F) are shown.