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. 2011 Jan 3;286(10):8165–8175. doi: 10.1074/jbc.M110.195255

FIGURE 6.

FIGURE 6.

SUMO modification is required for inhibition of type I IFN induction by PIASy. A, whole cell extracts from control or UBC9 knockdown (KD) 293T cells were analyzed by Western blot (WB) with the indicated antibodies. UBC9, α-tubulin (tub) and nonspecific bands (ns) are marked on the right. B, UBC9 KD or control 293T cells were transfected with WT or the activated form of IRF3 (left panel) or IRF7 (right panel) and IFNβ promoter reporter. Luciferase activities were quantified by normalizing with Renilla luciferase activities. C, UBC9 KD or control 293T cells were transfected with the activated form of IRF3 (left panel) or IRF7 (right panel) and IFNβ promoter reporter and increasing doses of PIASy. Luciferase activities were quantified as in B. The promoter activities are expressed as the ratio of PIASy-transfected versus untransfected cells. D, 293T cells were transfected with IRF3/5D (left panel) or IRF3/5D/K152R (right panel), IFNβ promoter reporter, and increasing doses of PIASy. Luciferase activities were quantified as in B.