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. 2011 Jan 1;286(10):8213–8230. doi: 10.1074/jbc.M110.165852

FIGURE 6.

FIGURE 6.

BID BH3 peptide neither interacts with BAKΔC in solution nor reproduces tBID-mediated activation of BAKΔC permeabilizing function. A, fluorescence polarization analysis of 21-mer FITC-labeled BID BH3 peptide (21 F-BID BH3) binding to BCL-XLΔC and BAKΔC is shown. Various concentrations of apoptotic proteins were incubated with 5 nm F-BID BH3 for 30 min followed by determination of fluorescence polarization. Data represent the mean (n = 3), with S.E. = 15% in all cases. B, ITC analysis of 29-mer BID BH3 peptide binding to BCL-XLΔC and BAKΔC. C, CL-enriched MOM-like LUV containing ANTS/DPX were treated for 20 min with the indicated protein/peptide combinations followed by determination of vesicular contents release. BAKΔC and BCL-XLΔC concentration were 200 and 1000 nm, respectively. tBID concentrations were 20 and 1000 nm in BAKΔC+tBID and BAKΔC+tBID+BCLXLΔC samples, respectively. Data represent the mean values and S.E. of at 3–5 independent experiments.