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. 2011 Jan 4;286(10):8231–8239. doi: 10.1074/jbc.M110.145888

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Association of SR-A with caveolin-1 or clathrin in RAW264.7 macrophages. A, coimmunoprecipitation of endogenous SR-A and clathrin. RAW264.7 cell lysates were incubated with an anti-SR-A specific or nonimmune goat IgG. The immunoprecipitated complexes were analyzed by Western blot for SR-A (SR-A) or clathrin heavy chain (cltc) as indicated. The presented blot is one of three independent blots. B, coimmunoprecipitation of endogenous SR-A and caveolin-1. RAW264.7 cell lysates were incubated with an anti-SR-A-specific or nonimmune goat IgG. The immunoprecipitated complexes were analyzed by Western blot for SR-A (SR-A) or caveolin-1 (cav1) as indicated. The presented blot is one of three independent blots. C, colocalization of endogenous SR-A and the caveolin-1 in RAW264.7 cells. Immunofluorescence experiments were performed by using antibody against SR-A (a, SR-A) and antibody against caveolin-1 (b, cav1) as indicated. c, merged images show overlapping subcellular distribution that appears yellow. D, colocalization of endogenous SR-A and clathrin in RAW264.7 cells. Immunofluorescence experiments were performed by using antibody against SR-A (a, SR-A) and antibody against clathrin heavy chain (b, cltc) as indicated. c, merged images show overlapping subcellular distribution that appears yellow. E, colocalization of endogenous SR-A and the caveolin-1 in RAW264.7 cells after incubation with fucoidan. RAW264.7 cells were incubated with 50 μg/ml fucoidan for 10 min. Immunofluorescence experiments were performed by using antibody against SR-A (a, SR-A) and antibody against caveolin-1 (b, cav1) as indicated. c, merged images show overlapping subcellular distribution that appears yellow. F, colocalization of endogenous SR-A and the clathrin in RAW264.7 cells after incubation with fucoidan. RAW264.7 cells were incubated with 50 μg/ml fucoidan for 10 min. Immunofluorescence experiments were performed by using antibody against SR-A (a, SR-A) and antibody against clathrin heavy chain (b, cltc) as indicated. c, merged images show overlapping subcellular distribution that appears yellow.