TABLE 1.
Bacterial strains used in this study and their relevant characteristics
| Straina | Relevant characteristics |
|---|---|
| A2594 | A:4:P1.9b:ST-5; invasive isolate (Germany) (Ref. 32); encapsulated; LOS not sialylated |
| A2594 fHbp NspA | Encapsulated; LOS not sialylated; fHbp::ermR; nspA::spcR |
| A2594 mynB fHbp NspA | Unencapsulated (mynB::cmR) isogenic mutant of A2594 fHbp NspA |
| H44/76 | B:15:P1.7,16: ST-32 (Ref. 65); invasive isolate; encapsulated; LOS sialylated |
| H44/76 lst fHbp NspA | Encapsulated; insertional inactivation of lipooligosaccharide (LOS) sialyltransferase (lst::kanR); LOS not sialylated; fHbp::ermR; nspA::spcR |
| H44/76 siaD lst fHbp NspA | Unencapsulated (siaD::cmR) isogenic mutant of H44/76 lst fHbp NspA |
| C2120 | C:NT:P1.5,2:ST-11 (Ref. 66); invasive isolate; encapsulated; LOS sialylated |
| C2120 lst fHbp NspA | Encapsulated; insertional inactivation of LOS sialyltransferase (lst::kanR); LOS not sialylated; fHbp::ermR; nspA::spcR |
| C2120 siaD lst fHbp NspA | Unencapsulated (siaD::cmR) isogenic mutant of C2120 lst fHbp NspA |
| W171c | W135:NT:P1.10:ST-11 (Ref. 26); invasive isolate; encapsulated; LOS sialylated |
| W171 lst fHbp NspA | Encapsulated; insertional inactivation of LOS sialyltransferase (lst::kanR); LOS not sialylated; fHbp::ermR; nspA::spcR |
| W171 siaD lst fHbp NspA | Unencapsulated (siaD::cmR) isogenic mutant of W171 lst fHbp NspA |
| Y2220 | Y:21:P1.15 (ST-172) (Ref. 26); carrier isolate; encapsulated; LOS sialylated |
| Y2220 lst fHbp NspA | Encapsulated; insertional inactivation of LOS sialyltransferase (lst::kanR); LOS not sialylated; fHbp::ermR; nspA::spcR |
| Y2220 siaD lst fHbp NspA | Unencapsulated (siaD::cmR) isogenic mutant of Y2220 lst fHbp NspA |
| LNP19995 lst fHbp NspA | W135:2a:P1.5:ST-11; invasive isolate; encapsulated; insertional inactivation of LOS sialyltransferase (lst::kanR); LOS not sialylated; fHbp::ermR; nspA::spcR |
| LNP19995 siaD lst fHbp NspA | Unencapsulated (siaD::cmR) isogenic mutant of LNP19995 lst fHbp NspA |
| Y2225 lst fHbp NspA | Y:NT:P1.2:ST-11 (Ref. 26); encapsulated; insertional inactivation of LOS sialyltransferase (lst::KanR); LOS not sialylated; fHbp::ermR; nspA::spcR |
| Y2225 siaD lst fHbp NspA | Unencapsulated (siaD::cmR) isogenic mutant of Y2225 lst fHbp NspA |
a All mutant strains were constructed for this study.
b Denotes that PorA VR typing (67) was used to define the serosubtype.
c W171 is also known as ATCC 35559, or M-603.