FIGURE 6.

RNA editing is required for appropriate male courtship. A, time taken to initiate courtship (latency) is significantly higher in dAdar^hyp males (n = 20) relative to dAdar^WTLoxP controls (n = 15), yet the total time spent courting virgin females over a 10-min period (courtship index, CI) is not significantly different between either genotype (B and C). Courtship index was either calculated over the whole 10 min (B) or following initiation of courtship (C). Examples of three separate song trains are shown from a single dAdar^WTLoxP (D) or dAdar^hyp male (E). Note that although the trains from the dAdar^WTLoxP male are highly stereotyped, trains from even a single dAdar^hyp male show striking variability in waveform pattern. Scale bar, 10 ms. F–H, song parameters in dAdar^WTLoxP (n = 26 songs, 5 males) and dAdar^hyp (n = 44 songs, 9 males). Error bars, S.E. values. *, p < 0.05; ***, p < 0.0005; not significant (ns): p > 0.05 (Mann-Whitney U test).