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. 2011 Jan 10;286(10):8375–8384. doi: 10.1074/jbc.M110.188789

FIGURE 5.

FIGURE 5.

STIM1-485-Ch coexpressed with Orai1 behaves like wild-type STIM1. A, STIM1-485-Ch was constructed by adding mCherry at the C-terminal end of STIM1-485. Coexpression of STIM1-485-Ch and Orai1 showed normal subcellular distribution of both proteins. Store depletion with TPEN led to co-clustering of STIM1-485-Ch and Orai1 in the focal plane of the cell membrane. Orthogonal sections are shown, with the focal plane of the images on the left indicated by the blue bar. Scale bar = 2 μm. B, time course of STIM1-485-Ch punctum formation as described under “Experimental Procedures” when coexpressed with Orai1 (mean ± S.E., n = 8). C, amplitude of ICl-T following the expression of full-length STIM1 (WT) or STIM1-485-Ch (485-Ch) with Orai1. D, ISOC mediated by coexpression of full-length STIM1 (Ch-WT) or STIM1-485-Ch (485-Ch) with Orai1. The SOCE development time course, current-voltage relationship, and amplitude are shown (n = 5–6).