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. 2010 Dec 29;286(10):8534–8544. doi: 10.1074/jbc.M110.165449

TABLE 2.

Binding affinities of nucleotide-Rv1739c STAS interaction

K½ values from fit of Rv1739c STAS fluorescence intensity quench by the indicated nucleotides at λem 316 nm.

Nucleotide K½ ± S.E. r2
μm
GTP 347 ± 227 0.96
GTPγS 326 ± 131 0.98
GTP + 1 mm MgCl2 727 ± 251 0.99
GDP 146 ± 38 0.98
GDPβS 152 ± 53 0.98
GDP + 1 mm MgCl2 587 ± 337 0.97
ATP 756 ± 232 0.94