FIGURE 9.

CTF forms dimers and binds to p53 and ERK. A–D, HCT116 p53^−/− cells were transiently transfected with the indicated plasmids. After 36–48 h of transfection, lysates were collected, pre-cleared and immunoprecipitated overnight with the indicated antibodies conjugated to protein G-Sepharose beads. Following extensive washing, beads were prepared for SDS-PAGE and immunoblotting with the indicated antibodies. A, cells were transfected with R175H p53 and various GFP-tagged constructs and immunoprecipitated with a monoclonal antibody against p53. B, cells were transfected with an HA-ERK1 expressing construct and the indicated GFP-tagged constructs and immunoprecipated with a monoclonal antibody against HA. C, cells were transfected with a plasmid expressing 3XFlagCTF and either GFP, GFP-CTF, or GFPΔ26CTF and immunoprecipitated with a monoclonal antibody against Flag. D, cells were transfected with R175H mutant p53 and either GFP, GFP-CTF, or GFPΔ26CTF. Lysates were immunoprecipitated with a monoclonal antibody against p53.