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. 2010 Dec 14;286(10):8585–8596. doi: 10.1074/jbc.M110.172411

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FIGURE 3. — Effect of monomer addition on fibril formation and toxicity of subfractionated protofibrils. A, ThT binding over time by 1:1 molar mixtures of Aβ42 protofibrils fractions (F1–F5) obtained from Superose 6 with Aβ42 monomers in supplemented 10× DMEM (10 μm Aβ42, 96 h of incubation; 37 °C, mean ± S.D.). B and C, cell viability (MTT reduction assay) of cultured PC12 and SHSY5Y cells after 24 h of treatment with 1:1 molar mixture of Aβ42 protofibrils fractions (F1–F5) and Aβ42 monomers (10 μm Aβ; one-way ANOVA, n = 9, *, p < 0.05; **, p < 0.01, mean ± S.D.). C, monomers were pretreated with IDE before addition to the fractions F1–F5 (Aβ:IDE, 20:1, w/w). D, cell viability (MTT reduction assay) of cultured PC12 and SHSY5Y cells 24 h after treatment with mixtures of sonicated Aβ42 fibrils (2 μm) with SEC protofibrils fractions F1–F5 (8 μm) (one-way ANOVA, n = 9; *, p < 0.05; **, p < 0.01, mean ± S.D.) (CR, crude Aβ42 protofibrils; a.u., arbitrary units; Veh, buffer vehicle).