FIGURE 7.
CHX sensitivity of two AtNug2 RNAi mutants. A, mutants AtNug2RNAi-5 and AtNug2RNAi-6 were constructed by inserting a 500-bp AtNug2 target sequence in sense and antisense orientations, and linked by a 300-bp partial GUS gene. The 35S-promoter (from the Cauliflower mosaic virus 35S-promoter) and OCS region are shown. B, RT-PCR analysis of AtNug2 expression in Col-0 and T2 progeny of RNAi transgenic plants. RNAs were isolated from whole plants 2 weeks after germination. The two RNAi transgenic plants show a marked reduction in AtNug2 transcript levels, but not in Actin2 expression. C, phenotypes of WT seedlings and RNAi mutants grown on MS/2 medium containing different concentrations of CHX. Ten-day-old seedlings germinated on MS/2 plates were transferred to CHX-containing medium and grown for a further 2 weeks. D, relative percentage FW of 2-week-old plants is dependent upon the concentration of CHX. Each data set represents the means of five independent measurements. Error bars represent the mean ± S.D.