FIGURE 8.

AtNug2 promoter-GUS expression patterns in transgenic Arabidopsis plants. The 1,500-bp AtNug2 gene promoter was inserted upstream of the GUS coding sequence in expression vector pCAMBIA1381. The AtNug2 promoter:GUS construct was introduced into A. tumefaciens strain LBL4404, which was then used to transform Col-0 plants by the floral dip method. Transgenic plants were selected and used for GUS activity assays. A, 30-day-old WT. B, 5-day-old whole seedlings. C, primary root tip of 5-day-old seedlings. D, meristematic regions of 5-day-old seedlings. E, 3-week-old whole seedlings. F, rosette leaf from a 3-week-old plant. G, lateral root tip from a 3-week-old plant. H, 3-week-old root stem and lateral root tip. I, flowers. J, mature silique. K, caulin leaf.