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. 2011 Jan 15;10(2):199–205. doi: 10.4161/cc.10.2.14444

Figure 2.

Figure 2

Mutation of equivalent CLS residues in cyclins A and E inhibits centrosomal localization. (A) Amino acid sequence alignment of the cyclin E1 and cyclin A2 CLSs in the conserved regions. Conserved residues are boxed in blue. Residues that lead to loss of CLS function when mutated into alanine are boxed in red. In the text, the resulting mutants are named SWNQ-A for cyclin E and DWVE-A for cyclin A. (B) Xenopus S3 cells were transfected with DNA constructs encoding either EGFP-fused-cyclin A CLS wild-type (Wt) domain (aa 201–255) or with a mutant CLS domain (DWVE-A) (upper parts). Lower parts, cells transfected with EGFP-fused-full-length (Fl) cyclin A with a wild-type CLS (wt) or mutant CLS (DWVE-A). Cells were methanol-fixed and stained for γ-tubulin as a marker for centrosomes, and EGFP was monitored by direct fluorescence. Arrows indicate the position of centrosomes. Insets: magnification of the centrosomal region in the merged image. Scale bars, 10 µm.