Skip to main content
. 2011 Jan 15;10(2):199–205. doi: 10.4161/cc.10.2.14444

Figure 3.

Figure 3

The mutated cyclin A CLS DWVE-A does not displace endogenous cyclin A from centrosomes or inhibit DNA replication. (A) CHO-K1 cells were transfected with DNA constructs encoding the EGFP- cyclin A CLS domain wt or carrying CLS loss-of-function mutations (IEEK-R and DWVE-A). The vector encoding EGFP alone was used as a negative control. Centrosomal localization of endogenous cyclin A in cells expressing these constructs was monitored and compared with the localization of endogenous cyclin A in cells expressing EGFP alone. Bars indicate mean ± SEM (n = 3). (B) CHO-K1 cells were transfected with DNA constructs encoding the Myc-cyclin A CLS domain wt or carrying CLS loss-of-function mutants (IEEK-R and DWVE-A). BrdU incorporation into DNA was monitored as a marker of S phase and compared with BrdU incorporation in cells expressing a non-centrosomally-localized cyclin A fragment (aa 1–200). Black bars represent BrdU incorporation in cells expressing the Myc-construct (Myc positive cells), whereas grey bars represent BrdU incorporation in cells not expressing Myc-constructs (Myc negative cells) from the same field. Bars indicate mean ± SEM (n = 3).